New Standard Operating Procedure: Measurement and Analysis of Mitochondrial Function In Situ in Permeabilized Muscle Fibers
We are excited to announce the release of our latest Standard Operating Procedure (SOP) titled “Measurement and Analysis of Mitochondrial Function In Situ in Permeabilized Muscle Fibers”, developed by our Basic Science Trainee Commitee members Dr. Sean Ng and Magda Lensinski. The SOP aims to provide a comprehensive, step-by-step protocol using the Oroboros O2k high-resolution respirometry system to precisely measure and analyze cellular oxygen consumption, mitochondrial function, and bioenergetic efficiency, particularly in dystrophic skeletal muscle.
This new resource provides detailed methods covering permeabilized muscle fiber bundle preparation, chamber calibration, experimental titrations (pyruvate, malate, ADP titration, glutamate, cytochrome c, and succinate), and data analysis using DatLab software. Researchers can use this protocol to assess mitochondrial respiration, oxidative phosphorylation, and metabolic flexibility in health and disease states.
Visit our SOP Library to check it out
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NMD4C Hosts 2nd Annual Clinical Summer School at UCalgary!
On September 24. 2026 the second annual NMD4C Clinical Summer School brought together 25 neuromuscular fellows from across Canada to the University of Calgary for a day of hands-on learning, discussion, connection, and collaboration. We like thank our program sponsors FUJIFILM Sonosite for their unwavering contribution to our annual program. We would also like to thank Summer School co-leads Drs. Marianne Nury and Gordon Jewett, as well as the workshop leads Drs. Grayson Beecher, Theodore Mobach, Christopher White and Shahin Khayambashi, for their time, expertise, and commitment to educating and supporting the next generation of neuromuscular clinicians.
2027 NMD4C–RARE.Qc Basic Science Research Summer School Applications Are Now Open!
Deadline: December 1, 2026
2027 NMD4C–RARE.Qc Basic Science Research Summer School Applications Are Now Open!
The summer school offers neuromuscular and rare disease research trainees the opportunity to participate in an intensive three-day training program focused on cutting-edge experimental and translational research techniques.
